May 1, 2017 Thesis Open Access

Tadele Tamiru

Citation Style Language JSON Export

{
  "DOI": "10.20372/nadre:5342", 
  "author": [
    {
      "family": "Tadele Tamiru"
    }
  ], 
  "issued": {
    "date-parts": [
      [
        2017, 
        5, 
        1
      ]
    ]
  }, 
  "abstract": "<p>Type 2 diabetes affect large population and able to develop micro and macrovascular complicati<br>\nons. There are many non- genetic and genetic factors or both are associated for the occurrence of<br>\ntype 2 diabetes mellitus (T2DM) and diabetic complications. Genes of the renin angiotensin syst<br>\nem angiotensin converting enzyme insertion/deletion gene polymorphism has been associated wi<br>\nth the risk of type 2 diabetes and its complications. The aim of the present study was to<br>\ninvestigate the association of angiotensin converting enzyme gene polymorphism, risk of type 2<br>\ndiabetes and its complications. A total of 222 subjects (111 T2DM and 111 healthy controls)<br>\nwere collected from Bahir Dar Felegehiwot referral hospital and Bahir Dar town, respectively by<br>\nusing non-probability purposive sampling techniques. Patients with T2DM were selected using<br>\nnon probability purposive sampling technique. Minidray fully automated analyzer was used for<br>\nbiochemical tests such as glucose, total cholesterol, triglycerol, urea and creatinine to assess the<br>\nassociated risk factors. The ACE I/D genotypes were identified by Polymerase chain reaction<br>\n(PCR) using appropriate primers and PCR reaction conditions. The present study revealed that<br>\nthe frequency of DD genotype and D allele were higher in type 2 diabetes mellitus compared to<br>\nhealthy controls (DD, 64.2% Vs 35.6% P &lt; 0 .001) and ( D, 79.3% Vs 59.9% P &lt; 0.001). DD<br>\ngenotype showed three fold increase risk towards T2DM as compared to II Genotype (OR:<br>\n2.984, CI: 1.332 - 6.689, P &lt; 0.02). The D allele carriers had five times high risk of getting<br>\ndiabetic as compared to I allele. (OR, D 2.178; CI: 1.168 &ndash; 3.232 P &lt; 0.001 Vs I OR, 0.459; CI:<br>\n0.309 &ndash; 0.681 P &lt; 0.001). To determine whether ACE gene polymorphism was associated with<br>\ndiabetic complication, patients with type 2 diabetes were divided into diabetic hypertension,<br>\ndiabetic retinopathy and diabetic nephropathy. The frequency of ACE DD genotype and D allele<br>\nin patients with diabetic hypertension were significantly higher than T2DM patients (41.6% and<br>\n60.4% Vs 19.5% and 37.8% P &lt; 0.01). The frequency of ACE DD genotype and D allele in<br>\npatients with diabetic retinopathy were also higher than T2DM patients (28.6% and 44.1% Vs<br>\n19.5% and 37.8%). The frequency of ACE genotype in patients with diabetic nephropathy did<br>\nnot show significant association</p>", 
  "title": "ASSOCIATION OF ANGIOTENSIN CONVERTING ENZYME GENE POLYMORPHISMS WITH RISK OF DIABETIC 2 AND ITS COMPLICATION AMONG PATIENTS VISITING BAHIRDAR FELEGEHIWOT REFERRAL HOSPITAL NORTH WEST, ETHIOPIA.", 
  "type": "thesis", 
  "id": "5342"
}