ISOLATION AND CHARACTERIZATION OF PHYTOCHEMICALS FROM THE ROOTS OF IMPATIENS TINCTORIA A. RICH AND INVESTIGATING THEIR ANTIBACTERIAL AND ANTIINFLAMMATION PROPERTIES

Medicinal plants have been a rich source of biologically active chemicals, which are widely employed in medicine as pure or as crude extracts to treat a wide range of illnesses. Impatiens tinctoria A. Rich has been utilized for a variety of medical purposes. The rich ethnobotanical information interests us to conduct phytochemical investigation on root of Impatiens tinctoria A. Rich. In this study, four compounds were isolated from the root of Impatiens tinctoria A. Rich using silica gel chromatography. The compounds were characterized by NMR and FTIR spectroscopy. The antimicrobial activities of the compounds and crude extract were evaluated against two Gram negative and two Gram positive bacteria by paper-disc diffusion method. Compound 21 didn’t show any activity against S. pyogenes. The other shows some antibacterial activities from lowest 7.43 mm by 20, against K. pneumoniae to maximum 9.6 mm by 23 against E. coli. The molecular docking of compound 21 against two human cyclooxygenases showed stronger binding affinity than two standard anti-inflammatory drugs (Flurbiprofen and (R)-naproxen).