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INVESTIGATION OF THE LEVEL OF VITAMIN C IN TEN WILD EDIBLE PLANTS CONSUMED IN NORTH SHOA ZONE, OROMIA REGION, ETHIOPIA

CHALCHISA ABEBE SORI


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    "description": "<p>Vitamin C also known as ascorbic acid is the main constituents of fruits and green leafy vegetables. It is an essential antioxidant. Presence of this organic acid plays an important role in human health. This study therefore intended to determine the level of vitamin C in selected wild edible plants such as Balanites aegyptiaca, Carrisa spinarium, Dovyalis abyssinica, Ficus mucuso welw, Opuntia ficus indica, Rubus apetalus, Syzygium giuneens sp., Thymus shimperi, Utrica simensis and Zzizphus spina-chriti which are commonly consumed around Fiche town. Acid hydrolysis method of sample preparation was used for plant sample preparation. 6% trichloro acetic acid solution was used as extraction solvent. In this method bromine water was used to oxidize ascorbic acid to dehydroascorbic acid. After coupling with 2, 4-dinitrophenyl hydrazine at 37 OC temperature for three hours, the solution was treated with 85% H2SO4 to produce a red color complex. Double beam UV-VIS spectrophotometer method was utilized for investigation of the level of vitamin C in wild edible plants and the absorbance was measured at 515 nm. Based on this method, it was investigated that the content of vitamin C was 0.175 &plusmn; 1.90 mg/gm to 1.612 &plusmn; 0.06 mg/g in investigated plants with percent relative standard deviation of 0.06 to 2.85% values (Utrica simensis 1.612 &plusmn; 0.06, Thymus shimperi 1.386 &plusmn; 1.44, Syzygium guineense sp. 1.274 &plusmn; 0.78, Doviyalis abyssinica 1.130 &plusmn; 0.84, Carissa spinarum 0.722 &plusmn; 1.38, Ziziphus spina-christi 0.531 &plusmn;1.88, Balanites aegyptiaca 0.402 &plusmn; 1.99, Rubus apetulas 0.350 &plusmn; 2.85 and \ud835\udc42\ud835\udc5d\ud835\udc62\ud835\udc5b\ud835\udc61\ud835\udc56\ud835\udc4e \ud835\udc53\ud835\udc56\ud835\udc50\ud835\udc62\ud835\udc60 \ud835\udc56\ud835\udc5b\ud835\udc51\ud835\udc56\ud835\udc50\ud835\udc4e 0.175 &plusmn; 1.14). Validation experiments revealed good percentage recovery of 98.14, good linearity with R2 -values 0.997 within the established concentration range. The LOD of the method was 0.05 mg/g whereas the LOQ was 0.15 mg/g. This study showed that the vitamin C contents of fruits and leaves sample varied with fruit and leaf type, and the vitamin C contents of leaves such as Utrica simensis and Thymus shimperi were higher than some domesticated green leafy vegetables such as cabbage and mustard spinach, moreover, the vitamin C contents of fruits such as Syzygium guineense sp., Dovyalis abyssinica and carissa spinarum were higher than some domesticated citrus fruits like lemon and orange. The levels of vitamin C in these wild edible plants were comparable with that of literature value. Therefore, it is recommended that considerable attention should be given to wild edible plants to supplement food insecurity.</p>", 
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    "title": "INVESTIGATION OF THE LEVEL OF VITAMIN C IN TEN  WILD EDIBLE PLANTS CONSUMED IN NORTH SHOA ZONE,   OROMIA REGION, ETHIOPIA", 
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