October 15, 2025 Thesis Open Access

AMANUEL UDESSA BIDIRE

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    <subfield code="a">Biological Nitrogen Fixation, Faba Bean, Nodulation, Rhizobia, Symbiotic  effectiveness, Uraga</subfield>
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    <subfield code="a">ISOLATION, CHARACTERIZATION OF PHENOTYPIC  AND SYMBIOTIC PROPERTIES OF RHIZOBIA  NODULATING FABA BEAN (Vicia faba L.) COLLECTED  FROM URAGA WOREDA, GUJI ZONE,  SOUTHEASTERN ETHIOPIA</subfield>
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    <subfield code="a">&lt;p&gt;&lt;strong&gt;ABSTRACT&lt;/strong&gt;: Faba bean (Vicia faba L.) is one of the most widely cultivated leguminous crops grown in the Highlands of Ethiopia. The symbiosis formed between leguminous crops and Rhizobia have paramount importance to enhance the productivity of the legume crops. The ability of indigenous rhizobia to nodulate a legume crop effectively was critical to successful establishment and growth of legumes. This study was aimed to isolate, characterize and evaluate the symbiotic effectiveness of rhizobia nodulating faba bean from Uraga Woreda, Guji Zone, and Southeastern Ethiopia. For this matter, a total of twenty five isolates of Rhizobium were isolated from thirty sampling sites of Uraga Woreda using plant infection method in Hawassa University, Soil Microbiology Laboratory. The rhizobial isolates were characterized morphologically and physiologically and tested on acid treated river sand to evaluate their symbiotic effectiveness. Results indicates that culturally almost all of them displayed large colonies with diameters of 1.5 to 5.0 mm, turned color of YEMA-BTB media into yellow, negative rod shaped and showed characteristics of fast growing rhizobia. The symbiotic effectiveness results on sand culture indicated that, the isolates showed shoot dry matter ranging from 0.35 (FBTB-2) to 1.43 g/plant (FBYT-2), with negative control of 0.44 g/plant and positive control of 1.26 g/plant. All the tested isolates were able to grow well within the ranges of 5.0-9.0, 0.5-2.0% and 150C-350C for pH, salt and temperature, respectively. The highest and lowest nodule number score was 67.00 (FBTHW-4) and 23.33 (FBDHB-5), respectively. Shoot dry weight was found to be strongly positively correlated with symbiotic effectiveness (r= 0.9597, P &amp;lt;0.001). The preliminary screening of the authenticated isolates for symbiotic effectiveness on sand culture showed 55.56% of the isolates were found to be effective, while 22.22% of the isolates viz. FBDHB-2, FBGG-3, FBTHW-4 and FBYT-2 were rated highly effective, of these FBGG-3 and FBYT-2 outcompeted KNO3 treated positive controls. Generally, the present work shows the physiological and symbiotic diversity of the isolates in the traditional agricultural areas of the study site and the potential of these rhizobia to be used as effective commercial inoculants in areas where the indigenous rhizobia fail to do so.&lt;/p&gt;</subfield>
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